Depletion of tissue mDCs followed by reconstitution after experimental peritonitis. (A) At different time points after CLP procedure, spleen and lung were collected and dispersed. The cells were stained with FITC–anti-CD11c, PerCP-Cy5.5–anti-CD11b and PE–anti-I-A^b. Myeloid dendritic cells (mDCs) were characterized as CD11c⁺CD11b⁺MHCII^hi. *P ≤ .05 compared with mDC number observed in spleen or lung from sham mice. Error bars represent SEM. (B) At day 11 after CLP procedure, spleens were collected and dispersed. Expression of CD40, CD80, CD86, and MHCII were investigated on the cell surface of CD11c⁺CD11b⁺ mDCs. Black line indicates isotype control; dashed line, sham group; gray line, CLP group.