Analysis of DC subsets in IRF8^R294C mice. (A) CD11c⁺ cells in spleen were analyzed for expression of the indicated DC subset markers by flow cytometry. (B) CD11c⁺ gated cells were tested for the pDC and IKDC markers, CD122 and PDCA1. (C) Total CD11c⁺ DCs per spleen. Values represent the average of 3 mice (± SD). (D) The percentages of 4 DC subsets in IRF8^WT and IRF8^R294C spleens. Values represent the average of 3 spleens (± SD). (E) BMDCs from IRF8^WT and IRF8^R294C mice grown in Flt3L were tested for B220 and CD24 as markers for pDCs and CD8α⁺ DCs, respectively. (F) BMDCs from IRF8^WT and IRF8^R294C mice were stimulated with CpG oligomer DNA D19 (1 μg/mL), LPS (100 ng/mL), or Poly IC (100 μg/mL) for 24 hours, and expression of CD8α was tested by flow cytometry. Numbers on plots are percentages of total cells.