LTR mediated LMO2 activation. (A) The relative LMO2 expression was compared by real-time qRT-PCR. All targeted clones showed a significant increase in LMO2 transcript over control Jurkat cells. M-3 and M-7 are single cell clones of untransduced Jurkat cells. K562 erythroleukemia cells in which LMO2 is constitutively expressed were used as the positive control. Values are expressed as percentages relative to the K562 control. (B) Expression of LMO2 protein was confirmed by Western blot analysis. Consistent with the qRT-PCR results, LMO2 protein was abundant in targeted clones but not in untransduced Jurkat cells. The upper portion of the Western filter was probed with an antibody to β-actin to provide a lane-to-lane loading control.