HGF and the HGF N-terminal domain, but not HGF kringles, bind to NRP1 and NRP2. ELISA analysis of (A) NRP1/Fc and (B) NRP2/Fc binding to immobilized ligands. Microtiter plates were coated with VEGF-A₁₆₅, HGF, N, K1, K2, K3, K4, or BSA and incubated with various concentrations of recombinant NRP1/Fc or NRP2/Fc, followed by the peroxidase-conjugated anti-Fc antibodies. Values are means plus or minus SD of 3 independent experiments.