HT-dependent protein sorting into clefts may occur at parasite periphery and is not influenced by deletion of the C-terminal domain of PfSBP1. (A) Three-dimensional projections of a live infected erythrocyte expressing HT-GFP^membmyc and stained with TR-ceramide. Clefts at the periphery of the infected erythrocyte (arrows) as well as at or within the perimeter of the vacuolar parasite (empty arrowheads) are visible. (B) 0° projection of live infected erythrocyte expressing HT-GFP^membmyc in 3D7 strains with parental (top) or chromosomal deletion of pfsbp1 (bottom), viewed under GFP optics and merged with bright field. Arrows indicate that the export of HT-GFP^membmyc to cleft structures in parental 3D7 strain is not altered in parasite line with a C-terminal deletion in PfSBP1. Parasite (p) nucleus is stained with Hoechst 33342. Bar, 2 μm.