Figure 2
The binding of sERGIC-53-SA-MCFD2 to DMJ- or SW-treated HeLaS3 cells. (A) HeLaS3 cells were cultured in the presence of 1 mM DMJ or 10 μg/mL SW for 24 hours. The binding of 20 μg/mL sERGIC-53-SA-MCFD2 (filled histograms) or PE-SA (thin lines) to the cells was measured by flow cytometry. The numbers in each panel indicate the MFI. (B) HeLaS3 cells cultured in the presence of 1 mM DMJ were incubated with or without 104 U/mL of endo H at 37°C for 3 hours. The cells were then mixed with 20 μg/mL PE-labeled sERGIC-53-SA-MCFD2 (filled histograms) or PE-SA (thin lines). The data shown are representative of 3 independent experiments with similar results.
