Distribution of EGFP⁺ cells in the livers from mice that had received clonal cell transplants with CCl₄ treatment. (A) Panel i shows EGFP as green and panel ii shows CD45 as red. Panels iii and iv show the merged images of EGFP, Alexa Fluor 568-labeled anti-CD45, and TO-PRO-3. Nuclei were stained with TO-PRO-3. White triangles indicate cells expressing both EGFP and CD45. White asterisks and open triangles show EGFP⁺CD45⁻ cells and EGFP⁻CD45⁺ cells, respectively. Bars equal 30 μm. Images were acquired using an Olympus IX81 FV1000 laser scanning confocal microscope equipped with 40×/1.30 NA oil-immersion objective lens and using FV10-ASW software (Olympus, Tokyo, Japan). Images were processed using Adobe Photoshop CS. (B) Panel i shows EGFP as green and panel ii shows F4/80 as blue. Panels iii and iv show the merged images of EGFP, Alexa Fluor 568–labeled anti-CD45, and APC-labeled anti-F4/80. White triangles indicate cells expressing EGFP, CD45 and F4/80. Open triangles show EGFP⁺CD45⁺F4/80⁻ cells. Bars equal 30 μm. Images were acquired and processed as in panel A. (C) The numbers of EGFP⁺CD45⁺ cells and EGFP⁺CD45⁺F4/80⁺ cells in the livers from 2 mice that had received clonal cell transplants. Data are means plus or minus a standard deviation.