Expression of vimentin and ORO in EGFP⁺ cells in the fibrotic liver. (Ai) EGFP as green. (ii) Vimentin as red. (iii,iv) Merged images of EGFP, Alexa Fluor 568–labeled anti-vimentin, and TO-PRO-3. Nuclei were stained with TO-PRO-3. White triangles indicate cells expressing both EGFP and vimentin. Bars equal 30 μm. Images were acquired and processed as in Figure 2A. (B) The numbers of EGFP⁺ cells and EGFP⁺vimentin⁺ cells in the livers from 2 clonal cells–transplanted mice. Data are means plus or minus a standard deviations. (C) Liver sections were stained with ORO (red in panel i; blue in panel ii) and Alexa Fluor 568–labeled anti-CD45 (red in panel iv). (iii) EGFP as green. (v) A merged image of panels ii, iii, and iv. EGFP⁺ORO⁺ cells (white asterisks) are negative for CD45. White triangles and open triangles indicate EGFP⁺CD45⁺ cells and EGFP⁻CD45⁺ cells, respectively. Bars equal 30 μm. Images were acquired and processed as in Figure 2A.