Characteristics of EGFP⁺ORO⁺ cells in the fibrotic liver. Sections were immunostained with anti-vimentin (A), anti-GFAP (B), or anti-ADAMTS13 (C), followed by Alexa Fluor 568–conjugated secondary antibody. EGFP⁺ORO⁺ cells are positive for vimentin, GFAP, and ADAMTS13. White triangles indicate cells coexpressing EGFP, ORO, and the respective markers. Open triangles indicate EGFP⁺ORO⁻vimentin⁺ cells (A) or EGFP⁺ORO⁻ADAMTS13⁺ cells (C). Bars equal 30 μm. Images were acquired using an Olympus IX81 FV1000 laser scanning confocal microscope equipped with 60×/1.42 NA oil-immersion objective lenses and using FV10-ASW software. Images were processed using Adobe Photoshop CS.