Characteristics of cultured nonparenchymal cells in the fibrotic liver. (A,E) Phase-contrast image merged with ORO (blue) of 3-day–cultured nonparenchymal cells, which were isolated from livers of mice that had received clonal cell transplants and were treated with CCl₄. (B,F) EGFP as green. (C,G) Immunofluorescent images with collagen I (C) and ADAMTS13 (G). (D,H) Merged images of ORO, EGFP, and collagen I or ADAMTS13. EGFP⁺ORO⁺ cells are positive for collagen I and ADAMTS13. Bars equal 30 μm. Images were acquired and processed as in Figure 4.