TRBV6-5 usage by DYS-specific CD4⁺ memory T cells. PBMCs from HLA-DRB*0701–positive CMV-seropositive subjects, donors 01 (A), 08 (B), 07 (C), and 11 (D) were stimulated with DYS peptide and stained for the expression of TRBV6-5 together with CD4 and intracellular IFN-γ. Histograms show TRBV6-5 expression by DYS-specific CD4⁺ cells (bold line) compared with an isotype control (narrow line). The percentage of CD4⁺IFN-γ⁺ cells expressing TRBV6-5 is shown.