Mature miRNA profiles of PEL. (A) Scatterplot analysis of 2 cell lines JSC-1 (vertical axis) and BC-1 (horizontal axis). Plotted are mean CT values of technical triplicates. Lines indicate the SD. Dots correspond to individual miRNAs. CT = 40 indicates absence of a miRNA signal. (B) Variation of mature miRNA levels in PEL. Plotted is the SD across multiple cell lines on the vertical axis against the mean CT on the horizontal axis (n = 10). Open circles indicate mature miRNAs as detected by TaqMan assay. Dark circles indicate that the corresponding pre-miRNA was also overexpressed. Crossed circles indicate miRNAs that were cloned from PEL (data from Cai et al17 and Samols et al18 ). Shaded area indicates the cutoff used to derive the PEL miRNA signature shown in Table S2. (C) Fraction of total (165) probes on the vertical axis, for which every PEL sample yielded a CT lower than indicated on the horizontal axis. All miRNAs with CT of 38 or less (ie, those that were uniformly detectable in all PEL lines) are tabulated on the right. The # indicates miRNAs that were also cloned once; ##, miRNAs that were cloned independently by 2 groups.17,18
