Sick Tg DCs maintain their antigen presentation and cytokine secretion capacity. CD11c⁺ spleen DCs from WT or sick Mushi1 Tg mice were magnetically purified. (A) Ova protein or peptide (cross)-presentation after 1-hour pulsing of irradiated DCs to OTI CD8⁺ T cells. Proliferation of T cells was measured by ³H-thymidine incorporation after 3 days of coincubation. (B) Ova or peptide presentation of irradiated DCs to OTII CD4⁺ T cells. Peptide or protein was left in the culture during the 3-day incubation period. Proliferation was measured as in panel A. (C) Secretion of indicated cytokines was measured by ELISA in supernatants of DCs from sick Mushi1 Tg mice that were activated with CpG, poly IC, IFN-γ, IL-4, and GM-CSF or not activated for 18 hours. The mean and standard deviation are shown for triplicate measurements.