Expression of wild-type ETV6 and wild-type RTN1. Expression analyzed by qPCR using primers exclusively targeting wild-type messenger RNA sequence for each gene. (A-B) Expression of wild-type ETV6 and RTN1 in germline/remission samples presented as “fold of control” [(E_target)^{ΔCPtarget(control-sample)}]/[(E_reference)^{ΔCPreference(control-sample)}]. The control value is calculated as an average of all 6 normal control samples analyzed and has been given the relative value of 1 (y = 1). (C-D) Expression of wild-type ETV6 and RTN1 in leukemic bone marrow presented as ΔCt (Ct[reference] – Ct[target]). In panel D, t(12;21) is not included due to lack of material for analysis.