Survival estimates of 60 patients whose MRD was analyzed integrating MFC and RT-qPCR. By integrating MFC and RT-qPCR, 4 categories of distinct MRD status were identified with different duration of OS (A) and DFS (B). Patients who were double negative had a 2-year OS and DFS of 89% and 69%, respectively. Patients who were MFCpos/PCRneg had a 2-year OS and DFS of 88% and 76%, respectively. Patients who were MFCneg/PCRpos had a 2-year OS and DFS of 87% and 65%, respectively. Finally, patients who were double positive had a 2-year OS and DFS of 55% and 22%, respectively.