Introduction of FE11 γδTCR in αβT cells can reestablish tumor cell recognition of clone FE11 cells. (A) To assess tumor reactivity, clone FE11 cells were incubated with SW480 or EBV-LCL tumor target cells. IFN-γ secretion was measured by ELISPOT. Healthy PBMCs served as negative control targets. (B) The TCR-γ and -δ chains of clone FE11 cells were sequenced and retrovirally transduced into αβT cells. Transfer of γδTCR-mediated tumor reactivity was tested by coincubating mock- (left) or γδTCR-transduced (right) T cells with indicated target cells in an IFN-γ ELISPOT. (C) The effect of blocking with FE11-like hybridoma supernatant on the recognition of SW480 and LCL-TM cells by FE11 γδTCR–transduced T cells. (D) LABScreen Single Antigen HLA class I beads were incubated with antibodies purified from hybridoma 6 (mAb6) or from hybridoma 12 (mAb12) and secondary α-mIgG-PE measured using Luminex. Error bars represent the standard deviation (SD; n ≥ 1). MFI, mean fluorescence intensity.