Activation of FE11 γδTCR–transduced T cells is dependent on expression of HLA-A*24:02. (A) Activation of T cells, transduced with FE11 γδTCR by EBV-LCLs with different HLA genotypes. (B) Activation of T cells, transduced with FE11 γδTCR by HLA-A*24:02 or HLA-A*02:01 target cells. (C) Activation of T cells, transduced with FE11 γδTCR by EBV-LCLs with different either homozygous or heterozygous HLA-A*24:02 expression. (D) Total HLA class I expression of HLA-A*24:02-positive and -negative EBV-LCLs compared with TEG011 recognition. (E) The effect of β2m knockout of HLA-A*24:02 target cells on the activation of FE11 γδTCR–transduced T cells. (F) Activation of T cells, transduced with FE11 γδTCR by K562 HLA-A*24:02 cells untreated or overnight monensin incubation. (G) Activation of Jurma cells, transduced with FE11 γδTCR or αβTCR WT1_126-134 (control) by LCL-TM or A2-restricted WT1_126-134 peptide–loaded T2 cells. CD3 cross-linking by plate-bound α-CD3 mAb clone OKT-3 served as the positive control. Recognition was assessed by measuring IFN-γ secretion using ELISA. Error bars represent the SD (n ≥ 1).