SR1 CD7⁺ cells generate a polyclonal T-cell repertoire. (A) Effective number of TCR clonotypes found in the spleen of naive CD7⁺ or SR1 CD7⁺ cell–engrafted mice, as determined by the True Diversity Index. Results are normalized to account for differences in the number of sequenced cells between conditions. (B) CDR3 spectratype for spleen-derived CD3⁺ cells from mice injected with naive proT cells or SR1 proT cells. The distribution of TCRα genes per CDR3 length is shown. (C) Heatmap representation of percentage of TRA V to J (oriented via chromosomal 5′ to 3′ distribution) pairings among total sequences in naive (left) and SR1 (right) T-lineage cells derived from the periphery of mice injected with either naive CD7⁺ cells or SR1 CD7⁺ cells. (D) TCRβ gene diversity in splenocytes from immunodeficient mice transplanted with day-14 naive or SR1 CD7⁺ cells.