Clonal architecture and types of mutational events, including ancestral and subclonal mutations. Somatic ancestral events are the only clones (A-B) followed by secondary events. (A) Linear evolution with a secondary subclone. (B) Branching evolution with 2 subclones. (C) Ancestral event occurs in germ line mutations and is nonclonal. The somatic hits are subclonal. (D) Codominant events occurring in close succession and thereby not distinguishable by VAF as a primary vs secondary hit.