Transactivation of the endoglin promoter by KLF6.

(A) Diagram depicting the endoglin promoter-reporter constructs is shown on the left. These reporter constructs were cotransfected with the KLF6 expression vector (▪) or the corresponding empty vector (■) in HeLa cells. Transcriptional activity was measured 24 hours later by the luciferase reporter assay and plotted as relative luciferase units (RLU). Shown is 1 of 4 representative experiments. Standard deviations are indicated. Numbers to the right of the closed bars indicate the -fold induction values after KLF6 transfection. (B) HMEC-1, M1, and Schneider 2 (SL-2) cells were transiently cotransfected with the indicated endoglin reporter constructs and the KLF6 expression plasmid, and the transcriptional activity was measured 24 hours later by the luciferase reporter assay. KLF6-transfected–sample-fold induction is expressed relative to cells transfected with an empty vector, whose arbitrary value is 1. The means of 3 different experiments (± SD) are shown in each panel.