Analysis of mice of all genotypes after a lethal hemolytic stimulus.

(A) Percent survival of wild-type, Hp-null, Hx-null, and HpHx dKO mice subjected to φhyd-induced hemolysis. Mice were injected with a single dose of φhyd of 0.2 mg/g body weight. Each group consisted of 60 mice. Differences between wild-type and single KO mice were not significant. Differences between wild-type and HpHx dKO mice were significant at each time point (P < .01). (B) Kidney sections of a wild-type (i,iii) and an HpHx dKO (ii,iv) mouse one day after an injection of 0.2 mg/g body weight of φhyd processed by immunohistochemistry with an anti-Hb antibody (i-ii) and by PAS staining (iii-iv). Note Hb deposition in proximal tubular cells in the wild-type mouse (more evident in the insert at a higher magnification of × 400) that results in tubular degeneration evidenced by brush border loss and flattened epithelium. Scale bars: i-ii, 100 μm; iii-iv, 50 μm.