Effect of addition of fibrinogen or VWF to AF or VWD blood on thrombus volume generated under high shear rate conditions.
Whole blood containing mepacrine-labeled platelets from a VWD or AF patient was perfused over a collagen surface for 7 minutes under high shear rate (1500 s−1) with or without addition of the respective deficient protein to blood prior to perfusion. Data represent the average (+ SD) total thrombus volume in a defined area (15 or 10 defined areas in control or patients, respectively, as described in Figure 1 legend). One-way factorial ANOVA and Scheffe method were used for analysis of variance and for comparisons with controls, respectively, with assistance of Stat View computer software (Abacus Concepts, Berkeley, CA). Asterisks indicate statistically significant differences from controls (P < .01). Statistical analyses demonstrated that the reduced thrombus volume in AF blood was only partially corrected by addition of purified fibrinogen (final concentration, 2.0 or 4.0 mg/mL) to AF blood, whereas the addition of purified VWF (20 μg/mL) to type 3 VWD blood completely normalized the reduced thrombus volume.
