FcεRI-dependent release of I-309 (CCL1), MCP-1 (CCL2), and MIP-1β (CCL4) by human and mouse cultured MCs.

Sensitized MCs were challenged with anti-IgE (panel A human, ▪), DNP-BSA (panel B mouse, ▪), or the control medium (■) and incubated at 5 × 10⁵ cells/mL. After 6 hours, the supernatants were collected. Each column and bar represents the mean and SEM of 6 (human) or 3 (mouse) experiments. The FcεRI-mediated production of MCP-1 was judged to be significant. I-309 and MIP-1β were below detectable levels in both supernatants and pellets of the cells incubated with the control medium, and the column represents the detectable level (#).