Cell surface expression of 4-1BB (CD137).

Cell surface expression of human (A) and mouse (B) 4-1BB is shown as the average values and SEs of the mean fluorescence intensity (MFI) values obtained by 3 independent experiments. (A) Human MCs were reacted with various concentrations of anti-IgE (as indicated) for 6 hours. The net MFI was obtained by subtracting the MFI given by isotype-matched control Ab (4.45-5.35) from the MFI given by Ab against 4-1BB. (B) Mouse MCs were reacted with 10 ng/mL DNP-BSA for 4, 8, and 24 hours. The net MFI was obtained by subtracting the MFI given by isotype-matched control Ab (3.19-5.29) from the MFI given by Ab against 4-1BB.