Quantitative analysis of TRAF1 mRNA levels.

Boxplot diagram of TRAF1 mRNA levels in B cells from healthy donors (left, n = 6), patients with B-CLL (center, n = 27), and 3 c-ALL cell lines (right). All measurements were done in duplicate by real-time PCR and compared with an internal cyclophillin control amplified in the same reaction. Duplicate experiments also involved independent reverse transcription. Δct ratios were calculated by subtraction of cyclophillin from TRAF1 values. For better illustration, TRAF1 ratios are presented as reciprocal values × 10¹. High ratios correspond to high TRAF1 mRNA levels. Depicted are the median (horizontal line), first and third quartiles (upper and lower edge of each box), 10% and 90% quantiles (vertical bars). The difference between healthy donors and patients with B-CLL was significant according to the log-rank test (P < .05).