American Society of Hematology

Fig. 6.

$Fig. 6. The absence of NK cells in tumor-bearing mice influences the composition and phenotype of adherent splenocytes. / Splenic cultures of control or tumor-inoculated mice were restimulated in vitro, and after 5 days the adherent fractions were analyzed. (A) The total number of CD11b+ adherent cells per restimulated spleen was calculated after measuring the expression of CD11b on the adherent CTL fraction via direct immunofluorescence and is represented for 5, 8, and 12 days of tumor-bearing state. Results are represented as means of 3 experiments ± SEM. (B) Adherent fraction of splenic cultures after 5 days of CTL restimulation. Samples used for this experiment were from the spleens of naive mice, tumor-bearing mice (5 days after tumor inoculation), and NK-depleted tumor-bearing mice (5 days after tumor inoculation). Morphologic appearance and FACS profiles of CD11b+ cells (open histograms) are shown. Isotype-matched monoclonal antibody was used as control for background staining (filled histograms). Original magnification × 200.$

The absence of NK cells in tumor-bearing mice influences the composition and phenotype of adherent splenocytes.

Splenic cultures of control or tumor-inoculated mice were restimulated in vitro, and after 5 days the adherent fractions were analyzed. (A) The total number of CD11b⁺ adherent cells per restimulated spleen was calculated after measuring the expression of CD11b on the adherent CTL fraction via direct immunofluorescence and is represented for 5, 8, and 12 days of tumor-bearing state. Results are represented as means of 3 experiments ± SEM. (B) Adherent fraction of splenic cultures after 5 days of CTL restimulation. Samples used for this experiment were from the spleens of naive mice, tumor-bearing mice (5 days after tumor inoculation), and NK-depleted tumor-bearing mice (5 days after tumor inoculation). Morphologic appearance and FACS profiles of CD11b⁺ cells (open histograms) are shown. Isotype-matched monoclonal antibody was used as control for background staining (filled histograms). Original magnification × 200.

This Feature Is Available To Subscribers Only