Effect of shear rate, soluble fibrinogen and blocking antibodies on erythrocyte adhesion to neutrophils.

(A) Erythrocyte adhesion to fibrinogen-adherent, fMLP-activated neutrophils was found to be inversely correlated to shear rate. Also, RBC adhesion to fMLP-activated neutrophils was more than 6-fold greater than to untreated neutrophils. (B) RBC adhesion to activated neutrophils was insensitive to soluble fibrinogen. (C) Comparison of percent adhesion with the control demonstrated significant reduction in erythrocyte adhesion to fMLP-activated neutrophils in the presence of anti-CD11b, anti-CD18, anti-LW, or EDTA. Data are expressed as means ± SD of n replicated experiments.