Phenotypic characterization of DCs cultured in GM-CSF/IL-4 and matured using CpG, TNF-α, or LPS and DCs cultured with Flt3L and matured using LPS.

Cultured DCs were harvested and washed with 2% FBS/PBS, blocked with αFCR, and incubated with directly conjugated fluorescent antibody for 30 minutes. Live cells were gated by using forward and side scatter dot plots, and 10 000 live events were analyzed for each sample. Data are presented as solid histograms for the expression of the phenotypic marker noted on the left for each preparation method; GM-CSF/IL-4 matured with TNF-α, LPS, or CpG 2006 or Flt3L matured with LPS. The dotted histogram represents the background fluorescence of the isotype control. Data presented are representative of 3 independent experiments.