Partially differentiated 5d-HSCs internalize bacteria.

(A) Phagocytosed L monocytogenes in 5d-HSCs (arrows; Giemsa staining at 1 hour after infection; bar, 10 μm). (B) Transmission electron microscopy reveals phagosomal bacteria (arrows) at 1 hour after infection (bar, 0.6 μm). (C) Fluorescence microscopic image. Detection of CD14⁺ partially differentiated HSCs (stained with anti–CD14-PE antibodies) and superimposed, intracellular green fluorescent L monocytogenes (yellow) carrying thegfp gene under control of the constitutively activesod promotor (1 hour after infection; bar, 10 μm). (D) Light microscopic image of the identical field (bar, 10 μm).