EBV protein expression in cells from an ulcerative lesion from patient no. 5.

Epithelial cells obtained from a genital lesion from patient no. 5 were dried onto poly-L-lysine–coated slides and fixed in a chilled 1:1 mixture of methanol and acetone, blocked with 20% normal goat serum, stained with primary antibody for BZLF1 (Argene) or LMP-1 (mouse monoclonal OT22C), then stained with fluorescein isothiocyanate (FITC)–conjugated goat anti–mouse IgG. Slides were mounted with coverslips using Vectashield (Vector Laboratories) and then subjected to confocal microscopy. Panel A shows staining with BZLF1 and panel B shows staining with LMP-1.