Changes in the expression level of TF mRNA in mouse tissues by restraint stress.

Eight-week-old C57BL/6J mice were placed into restraint tubes for 2 or 20 hours, and then the tissues were removed. Total tissue RNA was prepared and analyzed for TF mRNA expression level by quantitative RT-PCR assay as described in “Materials and methods.” For each tissue type, ■ indicates level before stress; ▨, after 2 hours of restraint stress; and ▪, after 20 hours of restraint stress. The data are represented as the means and SD (n = 8) in each amount of time under stress, and the error bars represent interanimal variation. *P < .05; **P < .02. Ki indicates kidney; Li, liver; H, heart; SI, small intestine; Ao, aorta; Adi, adipose (epididymal fat); Lu, lung; B, brain.