Induction of TF mRNA by restraint stress in the tissues of young and aged mice.

The indicated tissues were removed from 8-week- (8w), 12-month- (12m), and 24-month-old (24m) C57BL/6J mice before (open bars) and after (hatched bars) 20 hours of restraint stress. Total tissue RNA was prepared and analyzed for TF mRNA expression level by quantitative RT-PCR. The data are represented as the means and SD (n = 8) in each age group, and the error bars represent interanimal variation. *P < .05 in 24m versus 8w mice; **P < .04 in 24m versus 8w mice; ***P < .02 in 12m versus 8w mice.