In situ hybridization analysis of TF mRNA in the kidneys and small intestines of control and stressed mice.
Kidneys were harvested from 8-week-old and 12-month-old mice before and after 20 hours of restraint stress. Small intestines were also harvested from 8-week-old and 24-month-old mice before and after 20 hours of restraint stress. Both tissues were analyzed by in situ hybridization with the use of 35S-labeled cRNA probes as described in “Materials and methods.” The hybridization signal for TF mRNA corresponds to the light blue dots in panels B,D-H. (A-D) Kidneys of the unstressed (A, 8 weeks old; C, 12 months old) and stressed (B, 8 weeks old; D, 12 months old) mice. (E-H) Small intestines of the unstressed (E, 8 weeks old; G, 24 months old) and stressed (F, 8 weeks old; H, 24 months old) mice. Arrows indicate cells that are strongly positive for TF mRNA. M.M. indicates muscularis mucosae; V., villous core. All slides were exposed for 10 weeks at 4°C. Original magnification × 400.
