In situ hybridization analysis of TF mRNA in the aortas and adipose tissues of control and stressed mice.

Aortas and epididymal fat tissues were harvested from 8-week-old and 24-month-old mice before and after 20 hours of continuous restraint stress and then analyzed by in situ hybridization as described in “Materials and methods.” The hybridization signal for TF mRNA corresponds to the light blue dots in all panels. (A-D) Aortas of the unstressed (A, 8 weeks old; C, 12 months old) and stressed (B, 8 weeks old; D, 12 months old) mice. Arrows indicate cells that are strongly positive for TF mRNA in the adventitia of aorta. S.M. indicates vascular smooth muscle layer; Ad, adipose tissue around the vessel wall of aorta. (E-H) Epididymal fat tissues of the unstressed (E, 8 weeks old; G, 24 months old) and stressed (F, 8 weeks old; H, 24 months old) mice. All slides were exposed for 10 weeks at 4°C. Original magnification for all panels × 400.