Induction of TF mRNA by restraint stress in obese and lean mice.

Six-week-old male obese mice and their lean counterparts were stressed in restraint tubes for 20 hours. Mice were killed and the indicated tissues were removed. Total tissue RNA was prepared and analyzed for TF mRNA expression level by quantitative RT-PCR. The data are expressed as the means and SD (n = 8) in each phenotype. ■ represents level before stress; ▪, after 20 hours of restraint stress. *P < .05; **P < .04; ***P < .02.