Stress-induced thrombi formation in tissues of young and aged mice.

Renal and adipose tissues were removed from young (8-week-old) and aged (24-month-old) mice after 20 hours of restraint stress (n = 8 in each age group). Tissue sections were stained with PAS (A-B) or with hematoxylin/eosin (D-E) and examined microscopically. (A-B) Renal glomerulus of the stressed young (A) and aged (B) mice; original magnification, × 1000. An arrow denotes glomerular thrombus in aged mice. (D-E) Epididymal adipose tissues of the stressed young (D) and aged (E) mice; original magnification, × 400. An arrowhead indicates thrombi in the microvessels between adipocytes. Quantitation of stress-induced glomerular thrombi was achieved by counting the positive glomeruli for thrombi of at least 100 glomeruli in each kidney section from young (8w) and aged mice (24m), and the percentage of positive glomeruli for thrombi in each mouse is shown in panel C. Panel F shows each number of thrombi detected in the vasculature within the same size of microscopic area (90 mm²) in epididymal fat tissues of the stressed young (8w) and aged (24m) mice.