The association of DOCK2 and GST-CrkL-SH3(N).

(A) Lysates of human normal platelets were subjected to pull-down assay by GST (lane 1) or GST-CrkL-SH3(N) (lane 2). The precipitates were analyzed by immunoblotting with anti-DOCK2 (αDOCK2) Ab. Arrowhead indicates the size of DOCK2. (B) Lysates of Jurkat and MOLT4 were subjected to pull-down assay by GST (lanes 2 and 5) or GST-CrkL-SH3(N) (lanes 3 and 6). Arrowhead indicates the size of DOCK2. (C) Analysis of the binding of DOCK2 and various SH3 domains (indicated at the top) by pull-down assay in Jurkat cells lysates. The precipitates were analyzed by immunoblotting by means of anti-DOCK2 Ab (top panel) and anti-GST Ab (bottom panel). Lane 1, pull-down (−) indicates total cell lysates. In the bottom panel, asterisks indicate the expected size of each SH3 domain–containing proteins.