The uPAR-dependent cell adhesion to endothelial cells in AMI.

(A) MNCs from patients with AMI (n = 16) or CSA (n = 16) or from healthy volunteers (CON; n = 16) were pretreated with medium (white bars) or 10 ng/mL PMA (black bars) for 30 minutes at 37°C and washed, and adhesion to HUVEC monolayers was performed. Values shown are the means ± SEM from 16 independent experiments performed in triplicate. (B) Adhesion of MNCs from patients with AMI to HUVEC monolayers in the presence or absence of mAbs anti-CD18 (IB4; 20 μg/mL), anti-CD11b (CBRM1/5; 50 μg/mL), anti-CD11a (L15; 20 μg/mL), or different concentrations of anti-uPAR R3. Values shown are the means ± SEM from 3 experiments performed in triplicate. (C) MNCs from patients with AMI were pretreated with medium or pi-PLC (0.5 U/mL; 90 minutes) and washed. Adhesion to HUVEC monolayers was performed in the absence or presence of 20 μg/mL mAb anti-CD18 (IB4), anti-uPAR (R3), or anti-uPAR (R4). Values shown are the means ± SEM from 3 experiments performed in triplicate. The asterisks indicateP < .05.