Fig. 6.
Plasma from patients with AMI induces adhesion of MonoMac6 cells.
MonoMac6 cells were incubated with plasma (plasma:medium, 1:1 vol/vol) from patients with AMI or CSA or from healthy volunteers (CON; n = 3 in each group) for 60 minutes, and adhesion assays were performed. (A) Adhesion to immobilized fibrinogen. (B) Adhesion to immobilized vitronectin. (C) Adhesion to endothelial cell monolayers in the presence or absence of 20 μg/mL of blocking mAb anti-uPAR (R3) or anti-CD18 (IB4). Values shown are means ± SEM from 3 independent experiments performed in triplicate using plasma from 3 different patients or donors. The asterisk indicates P < .05.