HS mapping and C16orf35 gene expression.

(A) DNase I HS site mapping of the region around the HS −26 element in normal and homozygous HS −26 KO mice. A map of the area, with the position of the BamHI sites of the limit digest, is shown at the top. The bands in the KO sample are smaller than the normal bands because of the deletion of the HS −26 element. (B) Northern blot analysis of polyA⁺ RNA from embryos of WT mice and mice homozygous for the floxed HS −26 KO allele. The analysis used a probe for the C16orf35 gene and showed down-regulation of this gene in the floxed mice.