Diagram of experimental design.

PKH26-labeled Lin⁻ cells from C57BL/6J Ly5.1 mice were injected into the femur of primary recipients (F1 mice). At different times after injection, cells from different locations (injected femur, noninjected femur, and spleen) were harvested to determine donor cell distribution by FACS and to evaluate RU by competitive repopulation assay.