Proliferation profiles of donor cells in ablated and nonablated mice.
After injection of 5 × 105 Lin− Ly5.1 cells into the right femur, cells were collected from the injected femur, noninjected femur, spleen, and peripheral blood and were subjected to FACS analysis to determine the donor cell percentages. Two experiments were performed, with 5 to 8 mice per group. From 1 to 3 days, donor cells were identified by PKH26 labeling and antibody staining. After 3 days, donor cells were identified by PE-conjugated anti-Ly5.1 antibody. Donor cells rapidly proliferated in BM and spleen of ablated mice but did not proliferate in nonablated mice. The peripheral blood donor cell percentages also reflected this rapid proliferation of donor cells in ablated mice.
