Association of CLIP-170/restin with macropinosomes.

Monocyte-derived DCs pulsed with labeled dextran particles for 5 minutes to visualize macropinocytosis were stained for CLIP-170/restin after a 10-minute chase. (Top row) Immunofluorescence analysis of FITC-dextran–pulsed DCs (green) stained with a polyclonal rabbit anti–CLIP-170/restin serum (red) combined with DAPI labeling of nuclei (blue) reveals colocalization (yellow) of macropinosomes with regions of CLIP-170/restin enrichment. (Bottom row) Using confocal laser microscopy of DCs stained with monoclonal 2D6 antibody combined with FITC-conjugated secondary antibody (green) after pulsing with Texas Red dextran (red), the association of CLIP-170/restin with macropinosomes was confirmed.