Fig. 6.
TM11 increases HL60 rolling velocities in flow chamber perfusion experiments.
The rolling of calcein-am–labeled HL60 cells over CHO-P–coated coverslips was measured by real-time video microscopy in the presence or absence of peptides. Rolling velocities were calculated from the average rolling distance per elapsed time period. Rolling velocities were significantly enhanced by almost 2-fold in the presence of 100 nM (TM11)4 and DVEWVDVS (20 μM) compared with their controls [(Biotin)4 and DEAVDVS, respectively;P < .01]. Values represent means of rolling velocities of at least 20 cells in 3 separate experiments.