Fig. 1.
Fig. 1. Structures of the humanized (γ1, λ) CD3 mAb monomers and dimers and the reaction scheme for their preparation. / (A) WT indicates bivalent mAb not carrying the Ser444Cys mutation; H, heavy-chain polypeptide; L, light-chain polypeptide; HCys444, heavy-chain polypeptide carrying the Ser444Cys mutation; tHCys444, N-terminally truncated heavy-chain carrying the Cys233Ser and Ser444Cys double mutation; mal,N,N′-1,2-phenylenedimaleimide cross-linkage. (B) Outline of the stages involved in the dimerization procedure. DTT indicates dithiothreitol; Py-SS-Py, 2,2′-dipyridyl disulphide; o-PDM,N,N′-1,2-phenylenedimaleimide; G, blocking groups such as glutathione.

Structures of the humanized (γ1, λ) CD3 mAb monomers and dimers and the reaction scheme for their preparation.

(A) WT indicates bivalent mAb not carrying the Ser444Cys mutation; H, heavy-chain polypeptide; L, light-chain polypeptide; HCys444, heavy-chain polypeptide carrying the Ser444Cys mutation; tHCys444, N-terminally truncated heavy-chain carrying the Cys233Ser and Ser444Cys double mutation; mal,N,N′-1,2-phenylenedimaleimide cross-linkage. (B) Outline of the stages involved in the dimerization procedure. DTT indicates dithiothreitol; Py-SS-Py, 2,2′-dipyridyl disulphide; o-PDM,N,N′-1,2-phenylenedimaleimide; G, blocking groups such as glutathione.

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