SDS-PAGE analysis of CD3 mAb monomers and dimers performed under nonreducing conditions.

A 1.5-μg quantity of each sample was run on a 5% to 18% gradient polyacrylamide gradient gel possessing a 4% stacking gel, under nonreducing conditions. The gel was stained with Coomassie blue. Lane 1 (BM), bivalent^Cys444 monomer; lane 2 (BD), bivalent^Cys444 dimer; lane 3 (MM), monovalent^Cys444 monomer; lane 4 (MM), monovalent^Cys444 dimer; lane 5 (BM^WT), bivalent^WT monomer; MWM, molecular weight markers. The positions of the bands corresponding to the antibody monomers and dimers are illustrated on the gel.