![Fig. 5. MLL processing occurs within a few hours of its translation. / BOSC23 cells were transfected with empty vector (vector) or a construct encoding full-length MLL tagged with HA at its C-terminus [MLL(H)]. The cells were metabolically labeled with [35S]-methionine for 3 hours. After labeling, the cells were cultured in nonradioactive medium for the indicated time periods and then lysed in lysis buffer. MLL proteins were immunoprecipitated with anti-MLL antibody, separated in denaturing gels, and visualized by autoradiography (A and B) or immunoblotted with anti-HA (C and D) or anti-MLL N-terminal (E and F) antibodies. In the left panels (A, C, and E), the cells were harvested at 0, 3, and 9 hours after labeling. In the right panels (B, D, and F), the cells were harvested at 0, 1, and 2 days after labeling. Unprocessed MLL, the N-terminal fragment, and the C-terminal fragment are indicated by arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/10/10.1182_blood-2002-04-1015/4/h82223465005.jpeg?Expires=1735506217&Signature=C0cCdvDf7a-s62oAP~YBRMz9IX4McnKeau7N2WDgiHp66rXlY2FHeQi-4f5m8XQ2FXPvGCkS8NhOwvQX-9liT6nv~JW9j9Lq7XGmk3xcZwgUTIDQfRXt8huk0fgcBrP94kVHkByIQYFKeg~WEdl07dLJ4kkQNRUESa7KwiOKhsWlqO0obn5sx4B7OVm3qAuKPEyAdC53rLW2iiQsKuiDSCzMy1hB8U2eqH8sVIRw6dEuZ3EU0Vkp4xPZoMcXFlUmJVyFzS87SmI1g-JGImba4qge7ywKVUoP~hbMUtsZIOLeDW94gbg8DwuX6zFS638NhGswD0QtjXsy8yarepUggw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
MLL processing occurs within a few hours of its translation.
BOSC23 cells were transfected with empty vector (vector) or a construct encoding full-length MLL tagged with HA at its C-terminus [MLL(H)]. The cells were metabolically labeled with [35S]-methionine for 3 hours. After labeling, the cells were cultured in nonradioactive medium for the indicated time periods and then lysed in lysis buffer. MLL proteins were immunoprecipitated with anti-MLL antibody, separated in denaturing gels, and visualized by autoradiography (A and B) or immunoblotted with anti-HA (C and D) or anti-MLL N-terminal (E and F) antibodies. In the left panels (A, C, and E), the cells were harvested at 0, 3, and 9 hours after labeling. In the right panels (B, D, and F), the cells were harvested at 0, 1, and 2 days after labeling. Unprocessed MLL, the N-terminal fragment, and the C-terminal fragment are indicated by arrows.
