Fig. 7.
Fig. 7. Processing divides MLL into fragments with opposite transcriptional properties. / (A-C) Transcriptional properties of MLL and its cleavage products were evaluated as GAL4 fusion proteins. BOSC23 cells were transiently cotransfected with pFR-luc, pRL-tk, and expression vectors encoding GAL4-MLL 34/2666 (A), GAL4-MLL2719/3968 (B), or GAL4-MLL34/3968 (C). The cells were lysed 48 hours later and assayed for luciferase activity. The activity of each expression vector was divided by the luciferase activity of pLNCX-GAL4 to give the fold-activation. Results are presented as the averages ± SD of 2 independent experiments, each of which was performed in duplicate. (D) Expression of GAL4-MLL fusion proteins. BOSC23 cells were transfected with expression vectors indicated above the gel lanes, harvested 48 hours later, and analyzed by immunoblot with the anti-GAL4 antibody.

Processing divides MLL into fragments with opposite transcriptional properties.

(A-C) Transcriptional properties of MLL and its cleavage products were evaluated as GAL4 fusion proteins. BOSC23 cells were transiently cotransfected with pFR-luc, pRL-tk, and expression vectors encoding GAL4-MLL 34/2666 (A), GAL4-MLL2719/3968 (B), or GAL4-MLL34/3968 (C). The cells were lysed 48 hours later and assayed for luciferase activity. The activity of each expression vector was divided by the luciferase activity of pLNCX-GAL4 to give the fold-activation. Results are presented as the averages ± SD of 2 independent experiments, each of which was performed in duplicate. (D) Expression of GAL4-MLL fusion proteins. BOSC23 cells were transfected with expression vectors indicated above the gel lanes, harvested 48 hours later, and analyzed by immunoblot with the anti-GAL4 antibody.

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