Effect of OVA or PBS aerosol challenge on cellular composition of BALF.
Mice were immunized on day 0 with 1 × 106 OVA-DCs or PBS-DCs. From day 10 they were challenged daily for 30 minutes on 1, 3, or 7 consecutive days with OVA or PBS aerosols. (A) Total recovery of BALF cells as a marker of airway inflammation in response to aerosol exposures (*P < .05 compared with PBS-DC/PBS). Data are expressed as mean number of cells ± SEM. (B) Differential analysis of BALF cellular content based on flow cytometric analysis. The BALF composition of naive mice is included for reference. (C) Staining of BALF cytospin preparation with an Ab specific for murine MBP, identifying eosinophils in an OVA-DC immunized animal exposed 7 times to OVA aerosol. Isotype control Ab gave negative results (not shown). Original magnification, × 400. (D) Cells expressing high granularity on SSC signal (see gate) express the CCR3 receptor (filled histogram), identifying them as eosinophils. Isotype control is indicated by the open histogram.
