Effect of a dominant-negative Ras mutant (Ras-N17) on BCR/ABL- induced VEGF gene expression.

(A) Ton.B210-X cells induced to express BCR/ABL were transfected with Ras-N17 or a control plasmid (control) and were subjected to Northern blot analysis. The blot was probed for VEGF and reprobed for β-actin. (B) VEGF promoter activity. Ton.B210-X cells were cotransfected with VEGF-Luc/pCMV-βGAL and Ras-N17 or a control plasmid. Results represent the means ± SD of 3 independent experiments.